Knockdown of Sp1 (siSp1), Sp3 (siSp3) and Sp4 (siSp4) and their combination (siSp1, 3, 4) decreased growth, increased Annexin V staining (apoptosis) and decreased invasion in A549 lung, MiaPaca2 (pancreatic), SW480 (colon), 786-0 (kidney), SKBR3 (breast), MDA-MB231 (breast), Panc1 (pancreatic) and L3.6 pL (pancreatic) cancer cells. The gene discussed is SP3; the disease is cancer.