Here, we demonstrated that endometriosis in rats caused the activation of NLRP3 and NF-κB pathways as well as the increase in levels of IL-1β and TNF-α, while fisetin inhibited the activation of NLRP3/NF-κB as well as diminished the levels of IL-1β and TNF-α. The gene discussed is NFKB1; the disease is endometriosis.