RG Cq values were used individually [15], or the geometric means of the RQ values for three genes were combined [10,16]—PPP6R1, CCDC97, and ACTB, or PPP6R1, CCDC97, and EPHA2—to normalize TG Cq values to determine fold-change differences between the SM and ADM condition or the SM and OM condition by the comparative CT method (Figure 3, and Supplementary File S2). This evidence concerns the gene ACTB and ocular melanoma.