RG Cq values were used individually [15], or the geometric means of the RQ values for three genes were combined [10,16]—PPP6R1, CCDC97, and ACTB, or PPP6R1, CCDC97, and EPHA2—to normalize TG Cq values to determine fold-change differences between the SM and ADM condition or the SM and OM condition by the comparative CT method (Figure 3, and Supplementary File S2). The gene discussed is EPHA2; the disease is ocular melanoma.