The results showed that compared with the control group, the EGFRvIII-related pathways in U87-EGFRvIII (+) cells included cytokine–cytokine receptor interaction, tumor signaling pathway, PI3K-Akt pathway, etc., and in LN229-EGFRvIII (+) cells included cytokine–cytokine receptor interaction, tumor signaling pathway, TNF signaling pathway, etc. (Figure 5B). This evidence concerns the gene TNF and neoplasm.