MUC1.Tg mice were divided into five groups (n = 10 per group) and vaccinated with the following: (1) mock plasmid (pcDNA), (2) MUC1 plasmid (MUC1), (3) BMDCs (BMDCs), (4) pcDNA mixed with BMDCs (pcDNA + BMDCs), and (5) MUC1 + BMDCs, each followed by AOM-DSS treatment. This evidence concerns the gene MUC1 and infectious otitis media.