TGFB1 and breast cancer: We found that the mRNA (Figure 3D,E) and protein (Figure 3F) expression of TGF-β were downregulated in MDA-MB-231 and MDA-MB-453 cells treated with BC-siHSP70 CM compared with those treated with the siCTRL CM, although we could not detect TGF-β protein in the CM from MDA-MB-453 cells.