In order to observe the effect of the PI3K/AKT pathway on the expression of MMP7 and the ability of migration and invasion of hepatoma cells, MHCC97H and SMMC-7721 cells were treated with PI3K/AKT inhibitor LY294002 (20 μM) for 24 h. This evidence concerns the gene AKT1 and hepatocellular carcinoma.