The transcription factor (TF) motifs that were found to be upregulated with the highest statistical significance during EMT and that were enriched in the open chromatin regions of tumor mesenchymal-like cells (TMCs) were Jun/AP1 (42%), NF1 (45%), Ets1 (10%), bHLH TFs (20–45%), Nfatc (27%), and Smad2 (37%). The gene discussed is SMAD2; the disease is neoplasm.