Cellular assays showed that these compounds inhibited MYCN amplified human neuroblastoma cell lines with an IC50 of 26.5 μM for #5333 and 23.3 μM for #5338 in SK-N-BE(2)-C cells, and 28.6 μM for #5333 and 30 μM for #5338 in Kelly cells (Figure 1B). Here, MYCN is linked to neuroblastoma.