E-cadherin protein was upregulated, and N-cadherin was downregulated in SiHa shPRAME cells, while E-cadherin was downregulated and N-cadherin was upregulated in PRAME-overexpressed C33A cells (Figure 5A), suggesting that PRAME might affect cervical cancer metastasis by promoting EMT. The gene discussed is PRAME; the disease is cervical cancer.