The most upregulated genes in pediatric RUNX1/RUNX1T1-positive AML included RUNX1T1,LINC00189,M1AP,ADCY7,TPPP3,ADARB1, and TSPAN32, playing key role in ECM organization, protein polymerization, and cellular protein catabolic processes. The gene discussed is RUNX1; the disease is acute myeloid leukemia.