Although an earlier study demonstrated that miR-483-5p targets PCSK9, increases hepatic LDL Receptor expression, and ameliorates hypercholesterolemia in mice liver [34], our data suggest that mice fed with a high-fat diet and EtOH show downregulation of miR-483 expression compared with mice fed a regular diet, similar to our HCC tissue samples. Here, PCSK9 is linked to familial hypercholesterolemia.