Downregulation of miR-483 in the NAFLD mouse model increased TIMP2 and TGF-β expression, whereas down-regulation of miR-483 in the AFLD mouse model increased TGF-β expression but not TIMP2, indicating that miR-483/TIMP2 axis differentially regulated in fatty liver disease. This evidence concerns the gene TIMP2 and metabolic dysfunction-associated steatotic liver disease.