Similar results were obtained in an independent validation dataset of microarray-based mRNA levels for TGFB1, TGFB2, and TGFB3, and their correlated expression with specific transcription factors in tumor specimens from 41 pediatric patients with DIPG (n = 29) or H3K27M-mutant GBM (Figure 3). This evidence concerns the gene TGFB3 and diffuse intrinsic pontine glioma.