Altogether, this multicenter study conducted on a large number of samples representative of several dengue-endemic regions demonstrated a strong performance of the VIDAS® DENGUE NS1 Ag assay, either alone or in combination with the VIDAS® Anti-DENGUE IgM assay, notably at the early stage of DENV infection (first five days post symptom onset), and a strong performance of the VIDAS® Anti-DENGUE IgG assay at later stages of infection, either alone or in combination with the VIDAS® Anti-DENGUE IgM assay. This evidence concerns the gene CD40LG and infection.