After profiling DEGs between 438 HCC tumor samples and 45 CCA tumor samples, we observed down regulation of CCM2, PAQR9, and PGRMC1/2, along with AFP, in CCA tumor tissues, while CCM1, nPRs, and PAQRs5-8 were up regulated in CCA tissues (Figure 2(E-1) and Supplementary Table S2C). This evidence concerns the gene CCM2 and cholangiocarcinoma.