RASSF2 and cancer: Developed a method combining single-step sodium bisulfite modification and fluorescence PCR to measure RASSF2 and SFRP2 methylation status in fecal DNA.DNA recovery from feces showed an AUC of 0.78 for distinguishing cancer from non-advanced lesions (adenomas, polyps and healthy). Methylation levels showed an AUC of 0.78. A combination score showed the best AUC of 0.81.