B7-H3Dim EVs were mainly characterized by the expression of tetraspanin CD81 and by immune-related markers (i.e., CD56, CD3) whereas B7-H3Bright EVs expressed tetraspanin CD9 and an antigenic profile quite similar to the one characterizing PD-L1-EVs, with the exception of the tumor marker SSEA-4 [33] and CD69. This evidence concerns the gene CD81 and neoplasm.