DRAM1 and Miyoshi myopathy: To study the function of DRAM1 during Mm infection in macrophages, we generated three independent DRAM1 shRNA knockdown cell lines by lentiviral transduction, and showed that Dram1 mRNA expression levels (Supplementary Figure S5A), as well as protein levels (Supplementary Figure S5B), were reduced in each of the three knockdown cell lines compared to the control cells.