In addition, the viscous bile with abnormal pH causes bile salts accumulation and bile gallstones formation, though the CFTR function restoration in the biliary epithelium, with the new treatment, leads to increased bile fluidity that could trigger the movement of pre-existing gallstones and subsequently the appearance of biliary colic and/or acute or chronic cholecystitis [37,40,41,42,43]. This evidence concerns the gene CFTR and gallstones.