Treatment of PCOS mice with CGA indicated a substantial decrease in their levels, and the results are shown in Figure 4A. By using specific primers, we performed RT-PCR for the receptors of androgen, FSH, LH, and estrogen, and we observed that Ar, esr, and lhr were downregulated in LETZ-induced PCOS mice, while fshr was upregulated. The gene discussed is ESR1; the disease is polycystic ovary syndrome.