POU5F1 and embryonal carcinoma: Common characterization methods for pluripotency assessment in establishing iPSC lines include the detection of Nanog and Oct4, as well as the TRA-1-60 and TRA-1-81 cell surface antigens, that are expressed along with SSEA-3, SSEA-4 in human embryonic stem cells and embryonal carcinoma cells [24,93].