In order to confirm the generality of the Y407F instability and the importance of NEK1-mediated YAP1 phosphorylation, we stably transfected LNCaP PCa cells with GFP-YAP1-WT and GFP-YAP1-Y407F and examined YAP1 expression. The gene discussed is NEK1; the disease is posterior cortical atrophy.