GPT and metabolic dysfunction-associated steatotic liver disease: In addition to the ALT cut off values of 22 U/L (the median value) and 40 U/L (the upper limit of normal), we also analyzed our subjects by dividing them above or below 30 U/L because such a slight increase of ALT ≥30 U/L in Japanese subjects has been reported to be associated with lifestyle-related chronic liver diseases such as NAFLD [31].