Wan et al. [152] divided the experiment into two parts, in vivo and in vitro; for the in vivo experiments, mice were divided into DOX group (15 mg/kg, biw) and control group (normal saline, biw), and the results indicated the mice developed cardiac hypertrophy, and the expression of cardiac hypertrophy markers ANP and β-MHC was upregulated. The gene discussed is NPPA; the disease is cardiac hypertrophy.