TNF and colorectal cancer: Therefore, our research aimed to ascertain: firstly, whether antrodin C could inhibit the proliferation of HCT-116 cells by cell-cycle arrest and apoptosis; secondly, whether it is possible to define the precise mechanism of the inhibitory action through ROS/AKT/ERK/P38 signaling pathways and epigenetic histone acetylation of TNFα in colorectal cancer cells; and thirdly, an evaluation in vivo.