Therefore, we speculate that the best CAN efficacy on CFs on the 67.7 kPa PA gels is due to the most obvious decrease of p-FAK in CFs on the 67.7 kPa PA gels, which may be related to the expression of AT1R. We further characterized the expression of AT1R under the combined effects of ECM stiffness and CAN treatment. This evidence concerns the gene PTK2 and Crouzon syndrome-acanthosis nigricans syndrome.