Additionally, according to the statistical analysis of the grey values of α-SMA, MMP-2, type I, and type III collagen with and without CAN treatment (Figure 6D), the decreased degrees of the grey values on the 67.7 kPa PA gels are more obvious than those on the 29.4 and 125.5 kPa PA gels. The gene discussed is ACTA1; the disease is Crouzon syndrome-acanthosis nigricans syndrome.