CYBB and Crouzon syndrome-acanthosis nigricans syndrome: The NOX2 expressions of the rat heart tissues are up-regulated with the extension of ISO induction time, while the NOX2 expressions in the groups with CAN treatment are down-regulated (Figure 4A), which decrease by 21.6%, 26.7%, and 18.2% in the groups of ISO-1 W, ISO-2 W, and ISO-4 W, respectively (Figure 4B).