the expression of AT1R can be induced by ECM stiffness, and the expression level of AT1R increases with the increase of ECM stiffness, demonstrating the activation effect of ECM stiffness on AT1R. For the group with CAN treatment, CAN leads to the decrease of AT1R expression, which shows the opposite effect to the increase of AT1R expression induced by ECM stiffness. The gene discussed is AGTR1; the disease is Crouzon syndrome-acanthosis nigricans syndrome.