Figure 3E shows that LCB upregulated the expression levels of p21 and p27 but downregulated cyclin B1 and cdc2. Increased intracellular levels of ROS can lead to the apoptosis of cancer cells [15]. Therefore, we examined the intracellular levels of ROS after LCB treatment using the Muse® Oxidative Stress Kit. ROS levels were increased by 7.31 ± 0.46% (0 μM), 9.21 ± 0.87% (10 μM), 13.08 ± 0.77% (20 μM), and 36.56 ± 0.39% (30 μM) in HCT116 cells and by 8.10 ± 0.49% (0 μM), 12.99 ± 1.99% (10 μM), 22.67 ± 2.17% (20 μM), and 51.84 ± 1.47% (30 μM) in HCT116-OxR cells (Figure 3F). The gene discussed is CDK1; the disease is cancer.