In another study, the effect of naringenin, 17-estradiol (E2), and genistein on the activity of estrogen receptor (ER) in T47D-KBluc (cells containing the triplet reporter gene ERE (estrogen-responsive elements)-promoter-luciferase) and ER-negative MDA-MB-231 breast cancer cell lines was investigated. The gene discussed is ESR1; the disease is breast cancer.