Therefore, we detected the Hif2α protein and Epo mRNA levels in the kidneys, and the results confirm that the Irp2 KO-induced upregulation of Hif2α (Figure 3A,B) and increased mRNA of Epo returned to the proper level after administration of PT–2385 (Figure 3C), accompanying an improvement in anemia in Irp2 KO mice (Figure 1). This evidence concerns the gene EPAS1 and anemia.