Thus, this model has the following attributes: avoids the complex peaks and troughs of glycemic control that might occur in animals requiring exogenous insulin in terms of examining metabolic memory; eliminates background stimuli of the prior diabetic state and examines the pure epigenetic factors of metabolic memory; only takes about 80 days from diabetes induction to metabolic memory examination; allows easy genetic and experimental manipulation; allows for future drug discovery [11,71]. Here, INS is linked to diabetes mellitus.