To determine the effect of PCNA, MASP1, and BST2 on ASFV replication, Myc-PCNA, Myc-MASP1, or Myc-BST2 were transfected into MA104 cells, infected with ASFV (MOI = 1) at 24 h after transfection, and the processed samples were collected at 12, 24, 36, and 48 h post infection. Here, MASP1 is linked to infection.