The analysis indicated that the different proteins between LDEVs treatment group and control group were mainly enriched into MAPK signaling pathways, adrenergic signaling in cardiomyocytes, NOD-like receptor signaling pathway, Cushing syndrome, salivary secretion and bile secretion, insulin secretion, Relaxin signaling pathway, glycerophospholipid metabolism, cardiac muscle contraction pathway. The gene discussed is INS; the disease is Cushing syndrome due to macronodular adrenal hyperplasia.