Notably, our RNA-seq results suggested that Act1 knockdown in macrophage cocultured with CRC cells specifically enriched cytokines (IL-6, CXCL9/10, CXCL3, CXCL2, IFNβ1, TNF, IL-1β, IL-1α, etc.)expression related to TNF-α-NF-κB, cytokines-cytokines receptors, and IL-6-STAT3 signaling, suggesting the role of macrophagic Act1 in inflammation regulation. Here, TNF is linked to colorectal carcinoma.