Moreover, GSEA results from RNA sequencing showed that differentially expressed core genes in the anti-Act1 macrophages cocultured with CRC cells were enriched in the IFNα response, IL6-STAT3, TNFα-NF-κB, and IL-1R signal pathways (Fig. 3D (a-c)). This evidence concerns the gene TRAF3IP2 and colorectal carcinoma.