Notably, RT-qPCR analysis further confirmed higher expression of CXCL9/10, IL-6, Arg1, IDO1, and PD-L1 in anti-Act1 macrophages cocultured with CRC cells than wildtype macrophages cultured with CRC cells (Fig. 3E). The gene discussed is CXCL9; the disease is colorectal carcinoma.