However, the qPCR results showed that although the increase in Il-12 was not statistically significant, the expression of the proinflammatory cytokines Il-6 and Tnf-α was significantly higher in the CAR-M group than in the GFP-M group, and both the GFP-M and CAR-M groups exhibited increased Il-6, Il-12 and Tnf-α expression after coculture of engineered macrophages with MC38-HER2 cells (Fig. 3C–E), demonstrating that the proinflammatory phenotype was triggered by the interaction between macrophages and tumor cells rather than by lentiviral infection. This evidence concerns the gene ERBB2 and neoplasm.