The distribution of T cells contents and ESTIMATE scores was shown in Fig. 2B. It was found that the relative fraction of T cells CD8, T cells CD4 memory activated, T cells follicular helper and T cells regulatory (Tregs) was significantly higher in TCI cluster B than that in TCI cluster A, suggesting a higher T cell reactive activity in TCI cluster B. Moreover, Stromal score, Immune score and ESTIMATE score were also higher in TCI cluster B, indicating that TCI cluster B possessed the higher tumor purity. The gene discussed is CD4; the disease is neoplasm.