Analysis of colon cancer cohort in TCGA database showed that APC mutation was associated with a lower TMB and was associated with a lower genes expression of PDCD1 (PD-1), CD274 (PD-L1) and PDCD1LG2 (PD-L2) (Fig. 4A-F). This evidence concerns the gene PDCD1 and malignant colon neoplasm.