IRF8 and acute myeloid leukemia: Also, CRISPR-mediated depletion of NPM1c in OCI-AML3 cells markedly perturbed the CRC in AML cells expressing mtNPM1, with significant reduction in the CRC score and abolition of the contribution from the transcriptional regulators PU.1, IRF8, and RXRA to the CRC [7, 39, 40].