The recombinant green fluorescent protein (GFP) expressing the BCG Pasteur strain (rBCG-GFP) was used to examine the internalization ability of different murine DC subsets, and the infection rates (GFP-positive rate) of total DCs, cDC, pDC, and the CD8+ cDC and CD8− cDC subsets of the spleen were determined using flow cytometry (FACS) following intravenous (i.v.)administration of rBCG-GFP to mice (Figure 2A). Here, CD8A is linked to infection.