To understand if the efficiency of the combined treatment in sensitizingchemoresistant CRC cells was due to the regulation of the ABC transporterexpression, uL3ΔHCT 116p53–/– cellswere treated with 5-FU/NPs, β-car/NPs, or 5-FU/β-car/NPs.The total RNA was extracted after 24 h, and expression of ABCB1, ABCC9,and ABCC11 was analyzed by RT-qPCR with specific primers. Here, ABCB1 is linked to colorectal carcinoma.