Indeed, the liver ISR gene signature in mice fed HF.SAAR was augmented versus mice fed RF.SAAR, evidenced by Reactome pathways “eukaryotic translation elongation” and “response to eIF2AK4/GCN2 to amino acid deficiency” present only in the high-fat comparison (Fig. 3D, E). Here, EIF2AK4 is linked to hydrops fetalis.