Due to its three-dimensionality, the 3D cell culture model (forming multicellular aggregates—MCAs) shows important features for anti-cancer drug discovery and development, including the capability to mimic tumor characteristics in vivo such as cell morphology, gene expression, cell surface receptor organization, drug resistance, hypoxia, migration, proliferation, differentiation, dormancy, and anti-apoptotic patterns, as well as the cell-to-cell interaction and the tumor microenvironment including cell–extracellular matrix interactions [45,48,50]. The gene discussed is CD177; the disease is neoplasm.