Importantly, our results displayed the accumulation of autophagy-associated proteins (P62, Beclin 1, and LC3) in sEVs secreted by breast cancer cells treated with MA, but they were attenuated in sEVs derived from RIP1-transfected MDA-MB-231 and MCF-7 cells, indicating that autophagosomes can fuse with either lysosomes or MVBs, and further suggesting that the release of sEVs may compensate for lysosomal dysfunction. This evidence concerns the gene RIPK1 and breast cancer.