As macrophages play a key role in determining the activation or resolution of immune responses and can determine the fate of pathogen infection, we evaluated the robustness of our macrophage signatures (M1_IFNγ + LPS, M1_GM-CSF, M2_IL4 + IL10, M2_M-CSF) in discriminating macrophages infected with specific pathogens based on the enrichment analysis of selected genes. This evidence concerns the gene IFNG and infection.