A study found that the osteosarcoma LM-7 cell line, which showed highly invasive and metastatic features, markedly upregulated the expression of NOTCH1, NOTCH2, Dll1, and Hes1 compared with normal human osteoblasts and the Saos-2 cell line with low metastatic potential [17]. This evidence concerns the gene HES1 and osteosarcoma.