Similarly, Zhang et al. improved ovarian energy metabolism by regulating the glycolytic pathway through the co-administration of Diane-35 and metformin in PCOS symptomatic rats and found that the treatment upregulated SIRT1 expression and improved the regulation of ovarian energy metabolism by regulating two key glycolysis-related rate-limiting enzymes, PKM2 and LDH-A [41]. This evidence concerns the gene SIRT1 and polycystic ovary syndrome.