Compared with WT -AKI- CKD group, the levels of Synaptopodin, Nephrin, and CD2AP were significantly increased (P < 0.01) and the level of TRPC6 protein considerably decreased in the C3−/−-AKI- CKD group (P < 0.01) (Fig. 2d, e). Here, C3 is linked to chronic kidney disease.