Synaptopodin, Nephrin, and CD2AP immunohistochemical results showed a large number of brown-yellow granules in the glomeruli of the WT-sham group and C3−/−-sham group, while less in the WT-AKI-CKD group, and a moderate amount in the C3−/−-AKI-CKD group. The gene discussed is CD2AP; the disease is chronic kidney disease.