IRAG1 and hepatocellular carcinoma: 3I), which suggested that MRVI1-AS1 might be associated with the stability of SKA1 mRNA. Furthermore, actinomycin D assay was performed in MRVI1-AS1-related HCC subclones, then isolated RNA was subjected to RT-qPCR analysis. As expected, the half-life of SKA1 mRNA was dramatically shortened in the situation of deletion of MRVI1-AS1 (Fig. 3J), while the half-life of SKA1 mRNA was prolonged by overexpressed MRVI1-AS1 (Fig. 3K).