Additionally, the analysis of TCGA data from GEPIA platform consistently found the higher expression of MRVI1-AS1 in HCC (Fig. 1C), and RT-qPCR results in HCC cell lines revealed that MRVI1-AS1 expressions in all of the five HCC cell lines (Hep3B, Huh7, SK-HEP-1, HepG2, and MHCC-97H) were dramatically higher than that in the human normal liver cell line (LO2) (Fig. 1D). This evidence concerns the gene IRAG1 and hepatocellular carcinoma.