PFKFB3 and cancer: In this study, we proved the cancer-promoting effect of PFKFB3 in hyperglycemic breast cancer cells by regulating PFKFB3 expression starting from glycolysis pathway, but there are still some limitations: first, it might be inappropriate to simulate the hyperglycemic environment in the human body with hyperglucose mediums; second, the regulatory effects of PFKFB3 on RAS/MAPK pathway should be confirmed by co-immunoprecipitation assay; last, an hyperglycemic animal model should be established to further verify the results in vitro.