TOP2A and neoplasm: Specifically, this lineage suggests a scenario where naïve CD8+ T cells from lymph nodes or circulation were trafficking into the primary tumor with loss of circulating markers KLF2, SELL and CCR7, gain of tissue-resident marker CD103/ITGAE, progressive decline in the expression of naïve genes TCF7, IL7R, CCR7, and gradual gain of dysfunctional markers (TIM3, CTLA4, TIGIT, CXCL13, LAYN) with an intermediary proliferative burst with high levels of MKI67, TOP2A, TYMS (Fig. 4b, e, f).