We sought to assess physiological indicators of metabolic health and found that circulating insulin levels were much lower in the HFN group when compared to the HF group, with a major effect during the early active phase (Fig. 1d, ZT12-18, P < 0.001 Two-way ANOVA, Tukey post-test) and a six-hour phase delay in the oscillatory pattern (Supplementary Fig. S1f). This evidence concerns the gene INS and hydrops fetalis.