Furthermore, a GSEA comparing HF and HFN groups at ZT18 revealed highest enrichment in the hallmarks Cholesterol Homeostasis and MTORC1 Signaling (Supplementary Fig. S3c), with significant downregulation of transcripts coding for major regulatory proteins and rate-limiting enzymes triggered by de novo NAD+ oscillations (Supplementary Fig. S3d; Cholesterol Homeostasis: Hmgcr, Hmgcs1, Sqle, Acss2, Lss or Stard4; MTORC1 signaling: Acaca, Acly, Me1, Adipor2, Psma3, Psma4, Psmd14 or Psmc6). The gene discussed is ME1; the disease is hydrops fetalis.